hrp0097p1-373 | Sex Differentiation, Gonads and Gynaecology, and Sex Endocrinology | ESPE2023

Methodological considerations on determining sex steroids in children: Comparison of conventional immunoassays with LC-MS/MS

Ankarberg Lindgren Carina , Becker Charlotte , Svala Emilia , Ryberg Henrik

Objectives: In laboratory medicine, external quality assessment (EQA) schemes have become versatile tools for the detection of analytical flaws. However, for pediatric sex steroid levels EQA schemes are lacking. We aimed to investigate the suitability of different estradiol and testosterone immunoassays in a pediatric setting, in comparison with clinical liquid chromatography-tandem mass spectrometry (LC-MS/MS) assays.Methods:</s...